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| THE INFLUENCES OF TRANSPLANTED OLFACTORY ENSHEATHING CELLS ON AXONAL REGENERATION IN ADULT RAT SPINA |
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| Research from China | |||
| Samstag, 24. Dezember 2005 um 09:50 Uhr | |||
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There are no translations available. HY Shen, Y Tang, YF Wu, DH Zhou, L Huang, R Yang, P Wang, YP Shi and XY He Department of Orthopedics, Second Affiliated Hospital of Sun Yat-sen University; Institute of Spinal Cord Injury of Sun Yat-sen University, 107 Yanjiangxi Road, Guangzhou 510120 To observe whether olfactory ensheathing cells could be used to promote axonal regeneration in a spontaneously nonregenerating system, ensheathing cells was transplanted into a transected spinal cord. In primary cultures of adult rat olfactory nerve and glomerular layers (ONGL) of the olfactory bulb, ensheathing cells were purified away from other cell types. After laminectomy at the lower thoracic level, the spinal cords of adult rats were exposed and completely transected at T10. A suspension of ensheathing cells were injected into the lesion site in 12 adult rats, and control D/F-12 (1:1 mixture of DMEM and Ham’s F-12) were injected in 12 adult rats. Six weeks and ten weeks after cells transplantation, the rats were evaluated with climbing test and motor evoked potentials (MEPs) monitoring. At 6 weeks after cells transplantation, all rats in both of transplanted and control groups were paraplegic and MEPs could not be recorded. At 10 weeks after cells transplantation, 2 rats (n=7) had lower extremities muscles’ contraction, 2 rats had hips and/or knees’ active movement, and 5 rats’ (n=7) MEP could be recorded in the hind limbs in the transplanted group. None of the rats (n=7) in the control group had functional improvement and none of them had MEPs recorded. Numerous regenerating axons were observed grow through the transplant and continued to regenerate into the denervated host tract. Cell labelling using anti-Myelin Basic Protein (MBP) and anti-Nerve Growth Factor Receptor (anti-NGFR) indicated that the regenerated axons were derived from the appropriate neuronal source, and that donor cells migrated into the denervated host tract. But axonal degeneration exists and regenerating axons was not observed within the spinal cord of adult rats with only D/F-12 injection. In conclusion, the axonal regeneration in transected adult rat spinal cord was possible after ensheathing cells transplantation.
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