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| THE INFLUENCES OF TRANSPLANTED HUMAN FOETAL OLFACTORY ENSHEATHING CELLS ON AXONAL REGENERATION IN AD |
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| Research from China | |||
| Samstag, 24. Dezember 2005 um 10:02 Uhr | |||
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There are no translations available. HY Shen, Y Tang, YF Wu, DH Zhou, L Huang, R Yang, P Wang, YP Shi and XY He Department of Orthopedics, Second Affiliated Hospital of Sun Yat-sen University; Institute of Spinal Cord Injury of Sun Yat-sen University, 107 Yanjiangxi Road, Guangzhou 510120, The olfactory ensheathing cells (OECs) have attracted much interest recently because of their potential for transplantation-based therapy of spinal cord injury. Although rat OECs are able to support regeneration of damaged axons, it has been uncertain whether it would be similarly straight forward to these cells from the human embryo. In the present study, human foetal OECs (heOECs) were identified which share many properties with its rat counterpart, including expression of the low-affinity nerve growth factor receptor (L-NGFR) and similar growth factor requirements. In primary cultures of human foetal olfactory bulb (OB), heOECs were purified away from other cell types after 2 weeks culture. After laminectomy at the lower thoracic level, the spinal cords of rats were exposed and completely transected at T10. A suspension of heOECs was injected into the lesion site in 12 rats, and control D/F-12 (1:1 mixture of DMEM and Ham’s F-12) were injected in 8 rats. Two weeks and eight weeks after cells transplantation, numerous regenerating axons were observed grow through the transplant and continued to regenerate into the de-nervated host tract. Cell label using anti-Myelin Basic Protein (MBP) and anti-Nerve Growth Factor Receptor (anti-NGFR) indicated that the regenerated axons were derived from the appropriate neuronal source, and that donor cells migrated into the de-nervated host tract. But axonal degeneration existed and regenerating axons were not observed within the spinal cord of rats with only D/F-12 injection. Thus, heOECs transplantation would provide a new method for clinical therapy of spinal cord injury.
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| Zuletzt aktualisiert am Samstag, 24. Dezember 2005 um 10:03 Uhr |