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| Dr. Gao Shaorong - National Institute of Biological Sciences |
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| People | |
| Wednesday, 28 February 2007 02:29 | |
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Name: Dr. Gao Shaorong Ph.D. Assistant Investigator, NIBS, Beijing, China  Education 1993 B.S. Department of Animal Science and Technology, Shandong Agricultural University, Tai’an, China 1996 M.Sc Department of Animal Science and Technology of China Agricultural University, Beijing, China 2000 PH.D. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, China Professional Experience 2005-Present Assistant Investigator at National Institute of Biological Sciences, Beijing, China 2004-2005 Assistant Professor at University of Connecticut ,USA 2004 Associate Scientist at Fels Institute for Cancer Research and Molecular Biology , Temple University School of Medicine ,USA 2002-2004 Post-doctoral Scientist at Fels Institute for Cancer Research and Molecular Biology , Temple University School of Medicine , USA 2000-2002 Post-doctoral Scientist at Department of Gene Expression& Development of Roslin Institute, Britain 1998-2000 Research Associate at Division of Reproductive Medicine and Infertility Women or Infants Hospital of Rhode Island, Brown University School of Medicine , USA Research Description Our long-term goal is to understand the molecular mechanism of reprogramming during development of cloned embryos. The cloning efficiency remains very low with only 1-5% of cloned embryos can develop into offspring and the molecular mechanism of reprogramming of somatic cell genome remains unknown. We would be able to improve the cloning efficiency dramatically once the reprogramming factors present in the oocyte can be characterized, and it would be able to advance the stem cell research as well. Our previous research has demonstrated that the reprogramming factors are released from germinal vesicle during oocyte maturation. Our long-term aim is to define the factors present in the oocyte cytoplasm that are responsible for reprogramming of somatic cell genome after somatic cell nuclear transfer. Recently, embryonic stem cells are proposed as promising for therapeutic purpose. The ES cells can be derived from the cloned embryos reconstructed using the somatic cells from the patient which can eliminate the possibility of immune rejection after engraftment. We plan to establish somatic cell nuclear transfer ES (ntES) cell lines and further carry out the study on differentiation and functional research on the ntES cells in vitro. Gametes have been derived from the differentiation of embryonic stem cells in vitro recently. However, the gamete cells generated from embryonic stem cells are not functional and no offspring has been produced using these gamete cells. To produce functional gametes from embryonic stem cells would be another focus in my laboratory. Overall, our research will focus on: 1. Understand the molecular mechanism of reprogramming and define the reprogramming factors present in oocyte cytoplasm that are responsible for reprogramming of somatic cell genome. 2. Establish of ntES cell lines and study the differentiation and function of the ntES cells in vitro. 3. Generate functional gamete cells from embryonic stem cells. Publications 1. Sung L.Y.*, Gao S.*, Shen H., Yu H., Song Y., Smith S. L., Chang C. C., Kuo L., Lian J., Tian X. C., Tuck D. P., Weissman S. M., Yang X. and Cheng T.. Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer. Nat. Genet. 2006; 38(11):1323-1328. (* Co-first author) 2. Gao S.. Protocols for nuclear transfer in the mice. Methods in Molecular Biology. 2006; 325:25-33. 3. Chung Y.G., Gao S. and Latham K. E.. Optimization of procedures for cloning by somatic cell nuclear transfer in mice. Methods in Molecular Biology. 2006; 348: 111-124. 4. Miyara F., Han Z., Gao S., Vassena R. and Latham K. E.. Non-equivalence of embryonic and somatic cell nuclei affecting spindle composition in clones. Dev Biol. 2006; 289(1): 206-217. 5. Wu G., Hao L., Han Z., Gao S., Latham K. E., P. M. de Villena F, Sapienza C.. Maternal transmission ratio distortion at the mouse Om locus results from meiotic drive at the second meiotic division. Genetics. 2005; 170(1):327-334. 6. Gao S., Han Z., Kihara M., Adashi E., and Latham K. E.. Protease inhibitor MG132 in cloning: no end to the nightmare. Trends Biotechnol. 2005; 23:66-68. 7. Gao S., Wu G., Han Z., Casa-Esperon E. d. l., Sapienza C., and Latham K. E.. Recapitulation of the Ovum mutant (Om) phenotype and loss of Om locus polarity in cloned mouse embryos. Biol. Reprod. 2005; 72:487-491. 8. Nolen L., Gao S., Han Z., Chung Y. G., Bortolomei M, Latham K.E.. X chromosome reactivation and regulation in cloned embryos. Dev. Biol. 2005; 279: 525-540. 9. Han Z., Chung Y. G., Gao S., Latham K. E.. The maternal genome governs the earliest effects of the embryonic genome on embryo phenotype. Biol. Reprod. 2005; 72:612-618. 10. Gao S. and Latham K. E.. Maternal and environmental factors in early cloned embryo development. Cytogenetic and Genome Research. 2004; 105:279-284. 11. Gao S., Czirr E., Chung Y. G., Han Z. and Latham K. E.. Genetic variation in oocyte phenotype revealed through parthenogenesis and cloning:Correlation with differences in pronuclear epigenetic modification. Biol. Reprod. 2004; 70:1162-1170. 12. Gao S., Chung Y. G., Parseghian M. H., King G. J., Adashi E. Y., Latham K. E.. Rapid H1 linker histone transitions following fertilization or somatic cell nuclear transfer: Evidence for a uniform development program in mice. Dev. Biol. 2004; 266(1):62-75. 13. Gao S.. Nuclear transfer with murine embryonic stem cells. In: gene targeting and Embryonic Stem Cells (Advanced methods). (Eds: Jim McWhir and Alison Thomas). BIOS Scientific Publishers (Taylor & Francis Group). 2004; P23-43. 14. Gao S., McGarry M., Latham K. E. and Wilmut I.. Cloning of mice by nuclear transfer. Cloning and Stem Cells. 2003; 5:287-294. 15. Gao S., Chung Y. G., Williams J. W., Riley J., Moley K., Latham K. E.. Somatic cell-like features of cloned mouse embryos prepared with cultured myoblast nuclei. Biol. Reprod. 2003; 69:48-56. 16. Gao S., McGarry M., Ferrier T., Fletcher J., Harkness L., Sousa P. D. and Wilmut I.. Effect of donor oocytes and culture conditions on development of cloned mice embryos. Mol. Reprod. Dev. 2003; 66:126-133. 17. Gasparrini B., Gao S., Ainslie A., Fletcher J., McGarry M., Ritchie W. A., Springbett A. J., Overstrom E. W., Wilmut I. and De Sousa P. Cloned mice produced from embryonic stem cell karoplasts and activated cytoplasts prepared by induced enucleation. Biol. Reprod. 2003; 68: 1259-1266. 18. Gao S., McGarry M., Ferrier T., Pallante B., Gasparrini B., Fletcher J., Harkness L., Sousa P. D., McWhir J.and Wilmut I.. Effect of cell confluence on production of cloned mice using an inbred embryonic stem cell line. Biol. Reprod. 2003; 68:595-603. 19. Gao S., Gasparrini B., McGarry M., Ferrier T., Fletcher J., Harkness L., Sousa P. D. and I. Wilmut.. Germinal vesicle material is essential for nuclear remodeling after nuclear transfer. Biol. Reprod. 2002; 67:928-934.
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| Last Updated on Thursday, 05 February 2009 12:26 |