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| Stem Cell Transplantation; Allogenic chimeric mouse model indicate some donor stem cells exist more |
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| Research from China | |||
| Mardi, 13 Décembre 2005 08:00 | |||
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There are no translations available. Science Letter English An allogenic chimeric mouse model indicates that some donor stem cells exist in chimera for more than 6 months. According to recent research published in the Chinese Medical Journal, "It is essential to establish an animal model for the elucidation of the biological behaviors of stem cells in vivo. "We constructed a chimeric animal model by in utero transplantation for investigation of stem cell transplantation. This chimerism was achieved by injecting the stem cells derived from the bone marrow of green fluorescence protein (GFP)-transgenic mice into fetal mice at 13. 5 days of gestation." "Several methods such as polymerase chain reaction (PCR), real-time PCR, fluorescence-assisted cell sorting (FACS) and fluorescence in situ hybridization (FISH) were used for the observation of donor cells. Under a fluorescence microscope, we observed the GFP cells of donor-origin in a recipient. "PCR, FACS analysis and FISH indicated chimerism at various intervals. Real-time PCR indicated that some donor cells existed in chimera for more than 6 months," wrote M.L. Wang and colleagues at Shanghai Jiao Tong University. The authors concluded, "Allogenic stem cells may exist in recipients for a long time and this allogenic animal model provides a useful tool for studying the behavior of hematopoietic stem cells and also offers an effective model system for the study of stem cells." Wang and colleagues published their study in Chinese Medical Journal (Construction of an allogenic chimeric mouse model for the study of the behaviors of donor stem cells in vivo. Chin Med J (Engl), 2005;118(17):1444-1450).
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| Mise à jour le Samedi, 24 Décembre 2005 08:55 |