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Hui Zhen Sheng, See Xin Hua Hospital
Papers:
Long-term effects of bone marrow mononuclear cell transplantation on left ventricular function and remodeling in rats
Increased heme oxygenase-1 expression in infarcted rat hearts following human bone marrow mesenchymal cell transplantation
Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function
Bone marrow mononuclear cell transplantation into heart elevates the expression of angiogenic factors
Education and Professional Career:
1976 Medical Doctor, Shanghai Second Medical University, China
1989 PhD, La Trobe University, Melbourne, Australia
1989-2000 National Institutes of Health, Bethesda, Maryland, USA
Positions held:
Visiting Fellow
Visiting Associate
Senior Staff Fellow
Staff Scientist (Permanent position)
2000-present
Head, Shanghai Laboratory of Developmental Biology Shanghai Second Medical University
Shanghai
Major interests of research:
1) Human Stem cell and its medical application.
Embryonic stem (ES) cells can be propagated in culture indefinitely and remain pluripotent. Somatic cell cloning should also provide the possibility to produce ES cells that have a genetic background almost identical to which of the patient (therapeutic cloning). ES cells resulted from either conventional approach (Thomson JA et al. 1998 Science) or therapeutic cloning can then be directed to differentiate into particular cell types and transplanted back to the patient.
2) The molecular basis of pluripotency.
Publications (Selective):
1) Sheng HZ, et al; Early steps in pituitary organogenesis. Trends Genet, 1999 Jun, 15:6, 236-40
2) Zhao Y; Sheng HZ; Amini R; Grinberg A; Lee E; Huang S; Taira M; Westphal H., Control of hippocampal morphogenesis and neuronal differentiation by the LIM homeobox gene Lhx5. Science, 1999 May, 284:5417, 1155-
3) Takuma N; Sheng HZ (Co-first authors); Furuta Y; Ward JM; Sharma K; Hogan BL; Pfaff SL; Westphal H; Kimura S; Mahon KA, Formation of Rathke's pouch requires dual induction from the diencephalon. Development, 1998 Dec, 125:23, 4835-40
4) Sharma K; Sheng HZ (Co-first authors); Lettieri K; Li H; Karavanov A; Potter S; Westphal H; Pfaff SL., LIM homeodomain factors Lhx3 and Lhx4 assign subtype identities for motor neurons. Cell, 1998 Dec, 95:6, 817-28
5) Sheng HZ; Moriyama K; Yamashita T; Li H; Potter SS; Mahon KA; Westphal H., Multistep control of pituitary organogenesis. Science, 1997 Dec, 278:5344, 1809-12
6) Yamashita T; Moriyama K; Sheng HZ; Westphal H., Lhx4, a LIM homeobox gene. Genomics, 1997 Aug, 44:1, 144-6
6) Sheng HZ; Bertuzzi S; Chiang C; Shawlot W; Taira M; Dawid I; Westphal H., Expression of murine Lhx5 suggests a role in specifying the forebrain. Dev Dyn, 1997 Feb, 208:2, 266-77
7) Pichel JG; Shen L; Sheng HZ; Granholm AC; Drago J; Grinberg A; Lee, EJ; Huang SP; Saarma M; Hoffer BJ; Sariola H; Westphal H., GDNF is required for kidney development and enteric innervation. Cold Spring Harb Symp Quant Biol, 1996, 61:, 445-57
8) Pichel JG, Shen L, Sheng HZ, Granholm AC, Drago J, Grinberg A, Lee EJ, Huang SP, Saarma M, Hoffer BJ, Sariola H, Westphal H. Defects in enteric innervation and kidney development in mice lacking GDNF. Nature. 1996 Jul 4;382(6586):73-6.
9) Bertuzzi S; Sheng HZ; Copeland NG; Gilbert DJ; Jenkins NA; Taira M; Dawid IB; Westphal H Molecular cloning, structure, and chromosomal localization of the mouse LIM/homeobox gene Lhx5. Genomics, 1996 Sep, 36:2, 234-9
10) Sheng HZ; Zhadanov AB; Mosinger B Jr; Fujii T; Bertuzzi S; Grinberg, A; Lee EJ; Huang SP; Mahon KA; Westphal H Specification of pituitary cell lineages by the LIM homeobox gene Lhx3. Science, 1996 May, 272:5264, 1004-7
11) Sheng HZ; Lin PX; Nelson PG.,Combinatorial expression of immediate early genes in single neurons. Brain Res Mol Brain Res, 1995 Jun, 30:2, 196-202
12) Sheng HZ; Lin PX; Nelson PG.,Analysis of multiple heterogeneous mRNAs in single cells. Anal Biochem, 1994 Oct, 222:1, 123-30
13) Sheng HZ; Fields RD; Nelson PG., Specific regulation of immediate early genes by patterned neuronal activity. J Neurosci Res, 1993 Aug, 35:5, 459-67
14) Markwell MA, Sheng HZ, Brenneman DE, Paul SM. A rapid method to quantify neurons in mixed cultures based on the specific binding of [3H]ouabain to neuronal Na+,K(+)-ATPase. Brain Res. 1991 Jan 4;538(1):1-8.
15) Sheng HZ, Turnley A, Murphy M, Bernard CC, Bartlett PF. Epidermal growth factor inhibits the expression of myelin basic protein in oligodendrocytes. J Neurosci Res. 1989 Aug;23(4):425-32.
16) Sheng HZ, Kerlero de Rosbo N, Carnegie PR, Bernard CC. Developmental study of myelin basic protein variants in various regions of pig nervous system. J Neurochem. 1989 Mar;52(3):736-40.
17) Ying CHEN1,2 ,Zhi Xu HE3, Ailian LIU1,2, Kai WANG1,2, Wen Wei MAO1,2,Jian Xin CHU1,2, Yong LU1,2, Zheng Fu FANG1,2, Ying Tang SHI1,2, Qing Zhang YANG1,2, Da Yuan CHEN4, Min Kang WANG4, Jin Song LI4, Shao Liang HUANG3, Xiang Yin KONG5, Yao Zhou SHI5, Zhi Qiang WANG5, Jia Hui XIA6, Zhi Gao LONG6, Zhi Gang XUE6, Wen Xiang DING7, Hui Zhen SHENG1,2*.Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes Cell Research (2003); 13(4):251-264
18) Zhen Fu FANG1,2, Fan JIN3, Hui GAI1, Ying CHEN1, Li WU1, Ai Lian LIU1, Bin CHEN1, Hui Zhen SHENG1,2,*Human embryonic stem cell lines derived from the Chinese population. Cell Research (2005); 15(5):394-400
19) Qian Wang 1, Zhenfu Fang 2, Fan Jin 3, Yong Lu 1, Hui Gai 1, Hui Z Sheng 1* Derivation and Growing Human Embryonic Stem Cells on Feeders, Stem Cells 2005 Jun 13
Attachment:
About the Shanghai Laboratory of Developmental biology (SLDB)
Shanghai Laboratory of Developmental biology is established in August 1999 on campus of Shanghai Second Medical University (SSUM), with the mission to explore the potential of ES cells in regenerative medicine. The laboratory consisted of four units. A) Embryology unit, that studies development of germ cells and early embryos using systems such as oocyte culture, somatic cell reprogramming, and in vivo chimera formation et al. B) Stem cell unit, that focus on mechanisms regulating proliferation and differentiation of ES cells. C) Genes and Development unit, that works on inductive signals and transcription factors responsible for development of reproductive organs. D) Molecular Biology unit, that modifies the genome of embryonic stem cell for research or medical applications. The four units, enhancing each other, with the goal to explore the potential of human stem cells for medicine. A clinic extension of the laboratory is also currently under construction, which will host the future IVF center and a cell culture facility devoted solely for human stem cells.
From Jan. 1999 to present, the laboratory has achieved a series of scientific breakthroughs. We have established the technique to reprogram adult human somatic nuclei; the technique to derive embryonic stem cells from reprogrammed human blastocysts; the technique to differentiate embryonic stem cells into various cell types, such as muscle, adipocyte and hematopoietic stem cells et al. Thus, the laboratory has now equipped with a well-trained team and a technology platform efficient for the task of “therapeutic cloning”.
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